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. Author manuscript; available in PMC: 2010 Jul 28.
Published in final edited form as: Nature. 2010 Jan 6;463(7280):554–558. doi: 10.1038/nature08732

Figure 3. Knockdown of the elongator components Elp1 and Elp4 also impairs paternal DNA demethylation in zygotes.

Figure 3

(a) siRNA-mediated knockdown of Elp1 and Elp4 resulted in increased 5mC staining (green) in the PN5 paternal pronucleus. H3.3-mRFP1 (red) serves as a nuclear marker. ♂: male pronucleus, ♀: female pronucleus, PB: polar body. Bar = 25 μm. (b) Quantification of the ratio (male/female) of 5mC intensity in Elp1, Elp4, Elp3 knockdown and control groups. Each dot represents a zygote. Red bars represent the average ratio of each group. The statistics of the injections are presented in the table.