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. 2010 Mar 15;137(6):945–952. doi: 10.1242/dev.041657

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Fig. 3. — Generation of MMTV-Sim2s-HA transgenic mice. (A) Schematic diagram of the MMTV-Sim2s-HA targeting construct. The MMTV-LTR construct was provided by Dr Jeffery Rosen at Baylor College of Medicine. Exons 2 and 3 (E2, E3) and Intron 2 (I2) are derived from the rabbit β-globin gene (RβG). The poly-A signal is derived from the bovine growth hormone gene (bGHpA). (B)RT-PCR analysis of pooled samples from three transgenic females showing expression of the Sim2s-HA transgene exclusively in the mammary gland. Hrt, heart; Hyp, hypothalamus; Kid, kidney; Lng, lung; Lvr, liver; Neg, negative control (water); MG, mammary gland; Pit, pituitary; Ovr, ovary; SG, salivary gland; Spl, spleen; Utr, uterus; WAT, white adipose tissue. Gapdh was used as a loading control. (C) IHC analysis of HA localization in mammary glands from wild-type (WT) and transgenic (Sim2s-HA) V8 females.