Fig. 5.

Sim2s expression results in precocious expression of a subset of milk proteins and alveolar epithelial cell surface markers. (A-D) qRT-PCR was used to evaluate gene expression in samples from nulliparous wild type (WT) and transgenic (Sim2s) females (n=5 animals per group). (A) Csn2 expression. (B) Wap expression. (C) Lalba expression. (D) Expi expression. *, P<0.05. Cldn7 expression was used to normalize qRT-PCR data. (E) Immunohistochemical analysis of aquaporin 5 (Aqp5, top), the sodium-inorganic phosphate co-transport protein Npt2b (middle) and β-casein (bottom) in mammary glands from V8 WT and transgenic females.