Fig. 8.

Sim2s alters the binding of regulatory factors to the Csn2 promoter region. (A) Schematic diagram of the Csn2 gene. White boxes represent non-coding exons and black boxes represent coding exons (II-VIII). Arrows indicate the location of proximal promoter primers. (B) ChIP analyses of the proximal promoter were performed on chromatin isolated from UN HC11 cells or cells treated for 4, 8 or 24 hours with HC + Prl using the antibodies indicated to the left. (C) Re-ChIP analysis of Sim2s and RNAPII at the proximal promoter of Csn2 in control cells at PRL4. Following an initial pull down with Sim2s antibody, chromatin was re-immunoprecipitated with RNAPII antibody and the Csn2 promoter sequence was amplified using PCR. (D) ChIP analysis of Sim2s and AcH3 occupancy of Actnb coding region in control and Sim2s-overexpressing HC11 cells is included as a control.