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. 2010 Mar 15;137(6):913–922. doi: 10.1242/dev.043224

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Fig. 4. — Pigs can associate with both actin and microtubules. (A-B′) GFP-tagged full-length Pigs (GFP-PigsFL) expressed in the germline using nanosGal4-VP16 associates with microtubules near cell cortices (asterisks in B) and actin-based ring canals (arrow in B). (C-D′) GFP-PigsFL expressed in the FCs using Cy2Gal4 associates with microtubules. (E,E′) A GFP-tagged N-terminal version of Pigs containing the CH and Gas2 domains (GFP-PigsNT) expressed in the germline using nanosGal4-VP16 associates with actin-based ring canals (arrows in E) but not microtubules. (F-F‴) GFP-PigsFL expressed in late larval salivary glands using Intron2-Gal4 associates with both actin and microtubules at the basal surface of these huge epithelial cells, suggesting that it might have the ability to act as a cytoskeletal crosslinker.