Fig. 5.

Loss of Pigs leads to upregulation of Notch signalling in different tissues. Analysis of Notch signalling reporters in different tissues. (A-E) E(spl)m7-lacZ is expressed in the polar cells from ~stage 4 onwards in wild-type ovaries (A,B, arrows). In pigs¹ mutant ovarioles (C-E), expression is upregulated and also found in ectopic cells (arrows). (F,G) [Gbe+Su(H)]-lacZ labels anterior polar and border cells in the wild-type and main body FCs from stage 6 onwards. (H-J) In pigs¹ mutant ovarioles, levels of [Gbe+Su(H)]-lacZ are strongly increased in polar cells and main body FCs from stage 6 onwards. Also, ectopic activation in the invading and encapsulating FCs in the germarium is observed (I,J, arrowheads). (K-O) E(spl)mβ1.5-lacZ is expressed in the polar cells from ~stage 2-3 onwards in wild-type ovaries (K,L, arrows in L) but is strongly upregulated in all cells in pigs¹ mutant ovarioles (M-O, arrowheads in N,O). (P,Q) In the developing wing disc, E(Spl)m6-GFP labels the myoblast precursors. Insets show the whole disc and the large panel is a magnification of the myoblast region. In pigs¹ mutant discs, this labelling is strongly increased in level and number of cells labelled. (R) Schematic of Pigs as a direct target of Notch and possible feedback loops to Notch.