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. Author manuscript; available in PMC: 2010 Mar 8.
Published in final edited form as: Nat Immunol. 2002 Apr 22;3(5):451–456. doi: 10.1038/ni792

Figure 2. Signaling in X-CD40 transgenic mice relies on TRAF2/3 binding.

Figure 2

(a) Primary cultures of splenic B cells from unimmunized X-CD40 transgenic mice were stimulated in vitro with 1000 ng/ml of shCD154 for various times. Cells were lysed and lysates were immunoblotted (IB) with antibodies to the phosphorylated TpY motif of Jnk2. Both isoforms of phosphorylated Jnk, p54 and p46, were recognized by the primary antibody. (b) Analysis of p38 MAPK activation in primary splenic B cells that were treated with shCD154. Cells were stimulated for various times, then lysed and immunoblotted with an antibody to the phosphorylated TpY motif of p38 MAPK. (c) Analysis of NF-κB activation in primary splenic B cells. Cells were treated with shCD154 for various times, lysed and immunoblotted with an antibody to Ser32-phosphorylated IκBα. For all signaling experiments, data are representative of three or more independent experiments. (d) Measurement of cellular IκBα content in B cells from transgenic mice. The nitrocellulose membrane in c was analyzed by immunoblotting with antibodies to IκBα, as a control for equivalent protein loading.