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. Author manuscript; available in PMC: 2010 Mar 8.
Published in final edited form as: Cancer Res. 2009 Mar 10;69(6):2260–2268. doi: 10.1158/0008-5472.CAN-07-5575

Figure 2.

Figure 2

Lung metastases. Images were obtained at time of sacrifice from individual (numbered) mice in control and bicarbonate groups of the experiment shown in Fig. 1C. At time of necropsy, organ and lymph node green fluorescent tumor metastases from necropsies were detected by the Illumatool Bright Light System (LT-9500) using a 470 nm/40 nm excitation filter (Lightools Research) and imaged using a Stereomaster 4× dissecting microscope (Fisher Scientific) with mounted DC290 Zoom digital camera (Eastman Kodak). Images were captured at the same focal plane in the presence of 480-nm excitation and >490-nm filtered emission with an exposure time of 4 s for GFP images and 1/10 s for white-light illumination. Image data were analyzed with ImageJ (http://rsb.info.nih.gov/ij/) by segmenting the green channel and counting total positive pixels per field.