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. Author manuscript; available in PMC: 2010 Mar 8.
Published in final edited form as: Cancer Res. 2009 Mar 10;69(6):2260–2268. doi: 10.1158/0008-5472.CAN-07-5575

Figure 3.

Figure 3

Metastases and cathepsin B activity. A, at time of sacrifice, animals in the survival experiment shown in Fig. 1C were necropsied and metastases were quantified by fluorescence. Images were captured as described in Fig. 1C and fluorescence was quantified following RGB segmentation using ImageJ analysis software. Columns, average fluorescence pixel densities (fluorescence intensities × area) for lymph nodes, visceral organs, mesentery, and lungs; bars, SE. AUF, arbitrary units of fluorescence. B, red fluorescent protein–expressing MDA-MB-231 tumor cells were incubated at low and high pH values for 4 d, and then overnight in 0.2% serum media, followed by assessment of pericellular and intracellular cathepsin B activity in live cells via a “real time assay”, as described in Materials and Methods.