Table 1. Quantitative analysis of intravasation, extravasation, and pH.

	Control		Bicarbonate		P*
(A) Intravasation
	n	Mean (range)	n	Mean (range)
Manual (no. of cells)	3	0 (0)	7	1.3 (0–7)	NS
Fluorescence (AUF)	3	124 (70–180)	7	746 (0–4,358)	NS
Flow (cells/100 μL)	8	3.6 (0–13)	8	1.5 (0–10)	NS
(B) Extravasation
	n	Mean (SE)	n	Mean (SE)	P
Liver	3	109.0 (46.0)	3	11.9 (10.3)	0.007
Spleen	3	231.6 (3.6)	3	608.0 (56.0)	0.011
Ratio	3	0.54 (0.25)	3	0.035 (0.020)	<0.001
(C) pH and growth data from window chambers
	n	Mean (SE)	n	Mean (SE)	P
Intratumor pHe	4	7.00 (0.04)	3	7.07 (0.03)	0.19
Peritumor pHe	4	7.06 (0.00)	3	7.16 (0.03)	0.05
Distant pHe	4	7.11 (0.03)	3	7.15 (0.03)	0.14
ΔDiameter	4	−2.07 (13.32)	3	−5.88 (2.51)	0.39
ΔDensity	4	0.71 (0.26)	3	−3.29 (1.07)	0.002

NOTE: See Materials and Methods for experimental and analytic details.

Abbreviations: AUF, arbitrary units of fluorescence; NS, not significant.

^*P value from nonpaired Student's t test.