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. 2010 Feb;21(2):303–315. doi: 10.1681/ASN.2009070728

Figure 8.

Figure 8.

PKA can phosphorylate Ser-84 (UT-A1 and UT-A3), Ser-486-UT-A1, and Ser-499-UT-A1 in vitro. In each of the windows, the top half shows the reconstructed ion chromatogram for the phosphopeptide when PKA was not present in the reaction mixture; the bottom half shows the corresponding profiles for the phosphopeptide when PKA was added to the reaction mixture. (A) The C-terminal tail of AQP-2, which served as a positive control, is phosphorylated by PKA on Ser-256. Phosphorylation occurred only in the presence of kinase for (B) Ser-486-UT-A1, (C) Ser-84 (UT-A1 and UT-A3), and (D) Ser-499-UT-A1.