Table 1.
List of phosphopeptides that show significant change in abundance or no significant change with dDAVP treatment compared with control samples
| No. | Protein Name | Accession No. | Sequence (Charge) | Mean D/C | Log2 (D/C) | Sites |
|---|---|---|---|---|---|---|
| Part A | ||||||
| 1 | aquaporin 2 (collecting duct) | NP_037041 | R.QSVELHS*PQSLPR.G(+2) | 0.06 | −3.95 ± 0.24a | S261 |
| 2 | aquaporin 2 (collecting duct) | NP_037041 | R.RQSVELHS*PQSLPR.G(+3) | 0.10 | −3.39 ± 0.13a | S261 |
| 3 | aquaporin 2 (collecting duct) | NP_037041 | R.QSVELHS*PQSLPR.G(+3) | 0.11 | −3.20 ± 0.15a | S261 |
| 4 | ArfGAP with FG repeats 1 | NP_001129068 | K.SLLGESAPALHLNKGT*PT*QSPVVGR.S(+3) | 0.34 | −1.55 ± 0.27a | T177,b T179b |
| 5 | septin 9 isoform 2 | NP_789826 | R.LVDTLSQRS*PKPSLR.R(+3) | 0.41 | −1.30 ± 0.08a | S67b |
| 6 | ArfGAP with FG repeats 1 | NP_001129068 | K.SLLGESAPALHLNKGT*PT-QS*PVVGR.S(+3) | 0.44 | −1.19 ± 0.21a | T177, S181 |
| 7 | ysv oncogene homolog isoform A | NP_110484 | R.TIYVRDPT*S*NKQQRPVP-ES*QLLPGQR.F(+3) | 0.50 | −1.01 ± 0.37c | T37,b S38,b S48b |
| 8 | (ABC1), member 1 | NP_835196 | K.HVKAEMEQMALDVGLP-PS*KLK.S(+3) | 0.50 | −1.00 ± 0.49c | S964 |
| 9 | tensin like SH2 domain containing 1 | XP_341257 | R.WDSY*ENMSADGEVLHT-QGPVDGSLYAK.V(+3) | 0.51 | −0.98 ± 1.12c | Y410b |
| 10 | hsp 90, alpha (cytosolic), class A member 1 | NP_786937 | R.DKEVS*DDEAEEKEEK.E(+3) | 0.52 | −0.93 ± 0.82c | S231 |
| 11 | calpastatin isoform a | NP_445747 | K.NEAITGPLPDS*PKPMGID-HAIDALSSDFTCS*SPTGK.Q(+3) | 0.55 | −0.86 ± 1.15c | S260,b S280b |
| 12 | calpastatin isoform a | NP_445747 | K.NEAITGPLPDS*PKPMGID-HAIDALSSDFTCSS*PTGK.Q(+3) | 0.55 | −0.86 ± 1.15c | S260,b S281b |
| 13 | calpastatin isoform a | NP_445747 | K.NEAITGPLPDS*PKPMGID-HAIDALSSDFTCSSPT*GK.Q(+3) | 0.56 | −0.84 ± 1.13c | S260,b T283b |
| 14 | tensin | XP_237286 | R.HPVGSHQVPGLHSGVVTT-PGS*PSLGR.H(+4) | 0.60 | −0.73 ± 0.43c | S1523b |
| 15 | tx elongation factor A (SII) 1 isoform 2 | XP_001059640 | K.KKEPAISSQNS*PEAR.E(+3) | 0.62 | −0.70 ± 0.77c | S100b |
| 16 | tight junction protein 2 | NP_446225 | R.SQEES*PVPQPR.T(+2) | 0.62 | −0.69 ± 0.09a | S463 |
| 17 | nuclear RNA export factor 3 | XP_001053711 | K.S*SNINS*ILELFPK.L(+2) | 0.62 | −0.69 ± 0.23a | S287,b S292b |
| 18 | PDLIM1 interacting kinase 1 like | NP_001101454 | R.LDTS*DLEPT*LKVADFGLS-KVCS*ASGQNPEEPVSVNK.C(+3) | 0.62 | −0.68 ± 0.86c | S180,b T185,b S198b |
| 19 | (urea transporter), member 2 isoform 2 | NP_808877 | K.LYESELSSPT*WPSSSQDTH-PALPLLEMPEEK.D(+3) | 0.63 | −0.67 ± 0.93c | T37b |
| 20 | epsin 3 | NP_001019962 | R.TPVLPSGPPITDPWAPSSPT*PK.L(+2) | 0.63 | −0.66 ± 0.57c | T361b |
| 21 | (urea transporter), member 2 isoform 2 | NP_808877 | K.DLRS*S*DEDSHIVK.I(+3) | 0.63 | −0.66 ± 0.59c | S62, S63 |
| 22 | tensin | XP_237286 | K.VSS*SPVANGMAS*PSGSST-VSFSHTLPDFSK.Y(+3) | 0.63 | −0.66 ± 0.43c | S1667,b S1676b |
| 23 | calnexin | NP_742005 | K.SDAEEDGGTGS*QDEEDSK-PK.A(+3) | 0.64 | −0.65 ± 0.59c | S563b |
| 24 | AHNAK nucleoprotein isoform 1 | XP_001078032 | K.LEGEIKVPDVDISS*PGVNVE-APDIHVK.A(+4) | 0.64 | −0.65 ± 0.19c | S1807b |
| 25 | prostaglandin E synthase 3 (cytosolic) | NP_001124461 | K.DWEDDS*DEDMSNFDR.F(+2) | 0.64 | −0.64 ± 1.10c | S113b |
| 26 | beta-2-syntrophin | XP_001071043 | R.GLGPPS*PPAPPR.G(+2) | 0.64 | −0.64 ± 0.37c | S120 |
| 27 | PC4 and SFRS1 interacting protein 1 | NP_786941 | K.NLAKPGVT*STSDS*EEDDD-QEGEK.K(+3) | 0.66 | −0.60 ± 1.07c | T269,b S274b |
| 28 | tight junction protein 2 | NP_446225 | R.KVQVAPLQGS*PPLSHDDR.G(+3) | 0.66 | −0.59 ± 0.24c | S107b |
| Part B | ||||||
| 1 | AHNAK nucleoprotein isoform 1 | XP_001078032 | R.NRS*NS*FSDEREFSAPST-PTGTLEFAGGEGK.G(+3) | 1.51 | 0.59 ± 0.25c | S2454,b S2456b |
| 2 | AHNAK nucleoprotein isoform 1 | XP_001078032 | R.NRSNS*FS*DEREFSAPSTP-TGTLEFAGGEGK.G(+3) | 1.51 | 0.59 ± 0.25c | S2456,b S2458b |
| 3 | leucine-rich repeat (in FLII) interacting protein 2 | NP_001019932 | R.RGSGDTSS*LIDPDTSLSE-LR.E(+3) | 1.55 | 0.63 ± 0.29c | S138b |
| 4 | aquaporin 2 (collecting duct) | NP_037041 | R.RRQS*VELHS*PQSLPR.G(+3) | 1.55 | 0.63 ± 0.06a | S256, S261 |
| 5 | tankyrase 1-binding protein of 182 kDa | XP_215763 | K.RAS*VSTNQNTDENDQELR.M(+3) | 1.67 | 0.74 ± 0.18a | S883b |
| 6 | aquaporin 2 (collecting duct) | NP_037041 | R.RQS*VELHS*PQSLPR.G(+2) | 1.73 | 0.79 ± 0.17a | S256, S261 |
| 7 | PCTAIRE protein kinase 3 | NP_001093976 | R.RFS*MEDLNKR.L(+3) | 1.77 | 0.82 ± 0.05a | S66b |
| 8 | AHNAK nucleoprotein isoform 1 | XP_001078032 | R.NRS*NS*FSDEREFSAPSTPT-GTLEFAGGEGK.G(+4) | 1.91 | 0.93 ± 0.41c | S2454,b S2456b |
| 9 | aquaporin 2 (collecting duct) | NP_037041 | R.RRQS*VELHS*PQSLPR.G(+2) | 1.93 | 0.95 ± 0.21a | S256, S261 |
| 10 | calcium regulated heat stable protein 1 | NP_690003 | R.DRS*PS*PLRGNVVPSPLPTR.R(+3) | 2.28 | 1.19 ± 0.27a | S30, S32 |
| 11 | desmoplakin isoform I isoform 2 | XP_225259 | R.SMS*FQGIR.Q(+2) | 2.28 | 1.19 ± 0.77c | S2216b |
| 12 | PDZ and LIM domain 5 | NP_445778 | R.RGS*QGDIKQQNGPPR.K(+3) | 2.64 | 1.40 ± 0.42a | S228 |
| 13 | beta-catenin | NP_445809 | R.RTS*MGGTQQQFVEGVR.M(+3) | 2.69 | 1.43 ± 0.04a | S552b |
| 14 | beta-catenin | NP_445809 | R.RTSMGGT*QQQFVEGVR.M(+3) | 2.69 | 1.43 ± 0.04a | T556b |
| 15 | PCTAIRE protein kinase 3 | NP_001093976 | R.RFS*MEDLNK.R(+2) | 3.05 | 1.61 ± 0.21a | S66 |
| 16 | beta-catenin | NP_445809 | R.RTS*MGGTQQQFVEGVR.M(+2) | 3.29 | 1.72 ± 0.19a | S552b |
| 17 | aquaporin 2 (collecting duct) | NP_037041 | R.RQS*VELHS*PQS*LPR.G(+3) | 3.92 | 1.97 ± 0.07a | S256, S261, S264 |
| 18 | aquaporin 2 (collecting duct) | NP_037041 | R.RRQS*VELHSPQS*LPR.G(+3) | 4.14 | 2.05 ± 0.13a | S256,b S264b |
| 19 | plexin domain containing 2 | NP_001101892 | R.RGS*GHPAYAEVEPVGEK-EGFIVSEQC.-(+3) | 4.38 | 2.13 ± 0.24a | S507 |
| 20 | aquaporin 2 (collecting duct) | NP_037041 | R.RRQS*VELHSPQSLPR.G(+3) | 9.00 | 3.17 ± 0.20a | S256 |
| 21 | aquaporin 2 (collecting duct) | NP_037041 | R.RRQS*VELHS*PQS*LPR.G(+2) | 9.38 | 3.23 ± 0.39a | S256, S261, S264 |
| 22 | kinesin 13B | NP_998791 | R.RRS*S*GLQPQGAPEAR.R(+3) | 10.93 | 3.45 ± 0.20a | S1718, S1719 |
| 23 | aquaporin 2 (collecting duct) | NP_037041 | R.RQS*VELHS*PQS*LPR.G(+2) | 12.30 | 3.62 ± 0.41a | S256, S261, S264 |
| 24 | aquaporin 2 (collecting duct) | NP_037041 | R.RQS*VELHSPQSLPR.G(+2) | 14.93 | 3.90 ± 0.52a | S256 |
| 25 | (urea transporter), member 2 isoform 1 | NP_062220 | R.RKS*VFHIEWSSIR.R(+4) | 17.51 | 4.13 ± 0.22a | S486 |
| 26 | (urea transporter), member 2 isoform 1 | NP_062220 | R.RKS*VFHIEWSSIR.R(+3) | 19.84 | 4.31 ± 0.31a | S486 |
Mean and SE values are calculated from log2(D/C), where D and C are peak areas for dDAVP-treated and control samples, respectively. Phosphorylation sites are indicated with an asterisk.
aP < 0.05; significant change in abundance compared with control samples.
bAmbiguity in phosphorylation site assignment by the Ascore algorithm, despite certainty in phosphopeptide identification.
cNo significant change in abundance compared with control samples.