Figure 2.

Effect of ATP depletion on GSK3β, active Bax, caspase 3, and apoptosis is shown. Content of total and p-serine⁹ GSK3β, active Bax (6A7 epitope), total Bax, and β-actin in cells at baseline (‘Base’); after 60 minutes of metabolic stress (ATP Dep); and after 60 minutes of recovery (Recover) in cells that express empty vector (GSK3β S9A −) or constitutively active GSK3β (GSK3β S9A +). Immunoblot results are representative of two to three independent studies; each lane contains 40 μg of total protein.