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. 2010 Feb;21(2):284–294. doi: 10.1681/ASN.2009080828

Figure 4.

Figure 4.

Effect of GSK3β expression on renal cell survival after ATP depletion is shown. (A) Cell survival [3-(4,5 dimethylthiazol)-2,5-diphenyl tetrazolium bromide (MTT) assay] after 60 minutes of stress with 6 hours of recovery in cells expressing empty vector (EV), constitutively active GSK3β (GSK3β-SA9), or WT GSK3β (GSK3β-WT) compared with control at baseline. *P = 005 versus EV by ANOVA; n = 5. (B) Cell survival after 90 minutes of stress with 6 hours of recovery in control (CTL) or cells exposed to either nonspecific (NS RNAi) or specific (GSK3β RNAi) RNAi directed against GSK3β or to TDZD-8. *P < 0.05 versus EV by ANOVA; n = 5. None of the maneuvers significantly altered cell survival at baseline (data not shown).