Figure 6.

Effect of metabolic stress on Bax serine¹⁶³ phosphorylation is shown. (A) ³²[P] incorporation into a Bax-specific peptide containing serine¹⁶³, the residue targeted by GSK3β in a cell-free system (see the Concise Methods section), at baseline, immediately after 60 minutes of ATP depletion, and in presence and absence of 10 μM TDZD-8. *P < 0.05 TDZD-8 versus vehicle; n = 5. (B) Survival (MTT assay) 8 hours after 60 minutes of ATP depletion in cells exposed to nonspecific RNAi plus empty vector adenovirus (NS-si+EV), constitutively active GSK3β (GSK3β-S9A), Bax-specific RNAi (Bax-siRNA), or constitutively active GSK3β plus Bax specific RNAi (GSK3β-S9A+Bax siRNA). *P < 0.05 versus no ATP depletion; †P < 0.05 versus ATP-depleted CTL, no GSK3β-S9A versus GSK3β-S9A; ‡P < 0.05 versus NS-si+EV or GSK3β-S9A; §P > 0.05 versus Bax siRNA alone; n = 5.