Figure 5.

Uptake of peptides 1–8 in HeLa cells treated at 4 °C, 37 °C and under ATP-depleting conditions determined by FACS analysis. After incubation with 1.0 μM peptide, the cells were trypsinized and washed to remove any surface-bound peptide and subsequently had their fluorescence measured by flow cytometry. Each bar represents the fluorescence of 10,000 cells counted by FACS analysis and each experiment was performed in triplicate and the results are expressed as the geometric mean relative fluorescence ± standard deviation.¹²