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Scheme of electrophoresis based sulfotransferase assay. The assay mainly consists of four steps. First, spike sulfotransferase reaction with PAP35S. Second, separate the reactions on 8% SDS gel by electrophoresis and obtain an autoradiogram of the dried gel. Generation of the free sulfate is inevitable due to the degradation of PAPS. Third, cut out the hot spots on the dried gel and count the radioactivity. Fourth, data process.
