Table 1.
TCR format | Vector | +IPTG | -IPTG | +Sucrose | +IPTG +Sucrose |
---|---|---|---|---|---|
4B2A1 scTCR Vαβ | pHOG | 0.6b | 2.6 | 1.36 | 0.48b |
pFKPEN | 3 | 2.8 | 2.2 | 2.2 | |
pFKPEI | 2.8 | 2.8 | 2.1 | 2.4 | |
4B2A1 scTCR Vβα | pHOG | 2.6 | 2.6 | 2.2 | 2.2 |
pFKPEN | 2.8 | 3 | 2.5 | 2.4 | |
pFKPEI | 2.7 | 2.8 | 2.2 | 2.2 | |
7A10B2 scTCR Vαβ | pHOG | 3 | 3 | 2.6 | 2.4 |
pFKPEN | 0.8b | 2.8 | 2.2 | 0.58b | |
pFKPEI | 2.8 | 2.6 | 1.8 | 2 | |
7A10B2 scTCR Vβα | pHOG | 3 | 3 | 2.4 | 0.48b |
pFKPEN | 2.8 | 2.8 | 2.3 | 2.6 | |
pFKPEI | 2.8 | 2.8 | 2 | 2.2 | |
XL1-Blue cells without plasmid | 2.8 | 2.8 | 2 | 2.2 |
a A 600 nm after ON incubation of 5 ml expression cultures
b No growth of cultures after induction