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. 2010 Feb 3;10:8. doi: 10.1186/1472-6750-10-8

Table 1.

Growth characteristicsa

TCR format Vector +IPTG -IPTG +Sucrose +IPTG
+Sucrose
4B2A1 scTCR Vαβ pHOG 0.6b 2.6 1.36 0.48b
pFKPEN 3 2.8 2.2 2.2
pFKPEI 2.8 2.8 2.1 2.4

4B2A1 scTCR Vβα pHOG 2.6 2.6 2.2 2.2
pFKPEN 2.8 3 2.5 2.4
pFKPEI 2.7 2.8 2.2 2.2

7A10B2 scTCR Vαβ pHOG 3 3 2.6 2.4
pFKPEN 0.8b 2.8 2.2 0.58b
pFKPEI 2.8 2.6 1.8 2

7A10B2 scTCR Vβα pHOG 3 3 2.4 0.48b
pFKPEN 2.8 2.8 2.3 2.6
pFKPEI 2.8 2.8 2 2.2

XL1-Blue cells without plasmid 2.8 2.8 2 2.2

a A 600 nm after ON incubation of 5 ml expression cultures

b No growth of cultures after induction