Table 3.
Percentage of Gag CM9 positive cells quantified by imaging analysis of in situ stained lymphoid tissue sections.
| ID No | Specimen | Total cells/mm2 | CD8+ cells/mm2 | Gag CM9+ cells/mm2 | % Gag CM9 of CD8+ cells |
|---|---|---|---|---|---|
| RM 1 | Spleen | 16 678 (± 2 032) | 2 399 (± 386) | 164 (± 39) | 6.80 (± 0.6) |
| RM 2 | Spleen | 20 789 (± 3 552) | 2 048 (± 627) | 198 (± 70) | 9.59 (± 2.0) |
| RM 3 | Spleen | 20 276(± 3 877) | 2 694 (± 494) | 206 (± 49) | 7.78 (± 2.0) |
| RM 1 | Submandibular LN | 31 340 (± 4 076) | 3 333 (± 511) | 170 (± 11) | 5.21 (± 1.1) |
| RM 2 | Submandibular LN | 20 789 (± 3 552) | 4 885 (± 1 134) | 255 (± 78) | 5.16 (± 0.6) |
| RM 3 | Submandibular LN | 18 754 (± 3 317) | 3 684 (± 232) | 122 (± 22) | 3.31 (± 0.5) |
| RM 1 | Mesenteric LN | 23 330 (± 3 458) | 6 876 (± 1 225) | 262 (± 26) | 3.91 (± 1.0) |
| RM 2 | Mesenteric LN | 17 975 (± 2 866) | 2 969 (± 685) | 183 (± 48) | 6.51 (± 2.8) |
| RM 3 | Mesenteric LN | 21 588(± 3 470) | 6 476 (± 1 163) | 200 (± 42) | 3.26 (± 1.4) |
| RM 1 | Ileum | ND | ND | ND | ND |
| RM 2 | Ileum | 6 812 (± 2 603) | 701 (± 7) | 21 (± 3) | 2.97 (± 0.4) |
| RM 3 | Ileum | 6 793 (± 1 246) | 629 (± 206) | 15 (± 5) | 2.43 (± 0.3) |
| RM 1 | Colon | 10 049 (± 4 193) | 1 145 (± 135) | 77 (± 16) | 6.74 (± 0.9) |
| RM 2 | Colon | 11 814 (± 5 130) | 2 287 (± 1 424) | 76 (± 52) | 3.10 (± 0.5) |
| RM 3 | Colon | 11 126 (± 4 207) | 888 (± 160) | 42 (± 9) | 4.80 (± 0.9 |
The average total cell number/mm2, CD8+ cells/mm2, Gag CM9+ cells/mm2 and percentage of Gag CM9 Qdot 655 multimer positive cells in the CD8+ cell population was calculated by using the image J particle counting program. The standard deviation is shown in brackets. Staining was performed at least three times for each specimen and a total tissue area of 4.6 × 106 μm2 was counted for each specimen. LN; lymph node and ND; not done due to lack of material.