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. 2010 Feb 17;3:3. doi: 10.1186/1755-1536-3-3

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Copyright ©2010 Sokolović et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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IGFBP5 influences BCL2 expression. LX2 cells were transfected with control small interfering RNA (siCtrl) or IGFBP5 siRNA (siBP5). Sixteen hours after transfection the medium was replaced by serum-free medium. Recombinant IGFBP5 was added at t = 24 h and 45 h, after replacing the medium, to siIGFBP5 transfected cells (siBP5+rBP5). After 48 h RNA was isolated from control (siCtrl), silenced (siBP5), silenced treated with rIGFBP5 (siBP5+rBP5) and LX2 cells overexpression IGFBP5 (LX2(BP5)) and used for quantitative polymerase chain reaction in order to determine BCL2 mRNA. The BCL2 mRNA levels were normalized to 36B4 and given as a percentage of control cells.