Table 1.

LC-MS/MS SRM parameters and sensitivity details for NAD⁺ metabolites and related nucleosides and nucleotides

Metabolite	Parent mass	Product mass	Cone voltage	Collision Energy	RT (min)	LOQ (pmol)	R2	RSD (%)
Nam	123	80	35	20	3.68	0.45	0.996	7
NA	124	80	35	20	7.15	1.20	0.999	12
Cyt	244	112	35	10	4.52	0.10	0.995	10
Urd	245	113	90	10	4.56	1.20	0.978	10
NR	255	123	50	10	4.07	0.20	0.992	7
NAR	256	124	35	10	4.80	0.06	0.984	5
Ino	269	137	80	10	6.31	0.07	0.993	3
CMP	324	112	35	10	12.05	0.68	0.999	6
UMP	325	97	90	15	12.22	0.21	0.989	8
NMN	335	123	35	10	9.04	0.50	0.988	9
QA	166 (-)	122	35	10	12.34			9
NaMN	336	124	35	10	11.66	0.18	0.999	5
IMP	349	137	80	10	13.59	0.13	0.997	5
NAD+	664	542	35	18	8.69	0.17	0.996	8
NaAD	665	542	35	20	11.59	0.26	0.987	8
NADH	666	649	35	20	11.77	0.06	0.991	16
NADP	744	604	35	25	14.01	0.87	0.995	9
NADPH	746	729	35	20	16.18	0.17	0.994	8