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EMSAs of CUP-1 and CUP-2 binding site probes with preadipocyte and adipocyte nuclear extracts. Nuclear extracts from 3T3-L1 adipocytes (lanes A) or preadipocytes (lanes P) were incubated either with a 32P-labeled CUP-1 site or CUP-2 site oligonucleotide in the absence or presence of unlabeled competitor CUP-1, CUP-2, or mutated CUP-1* site oligonucleotides. After incubation, reaction mixtures were subjected to TBE/polyacrylamide gel electrophoresis and radioautography.
