Table 1.
Spleen cell source | Stimulant | Amount (μg) | Absorbance |
---|---|---|---|
Normal | MSP-119 | 10 | 0.090 |
Normal | MSP-119 | 20 | 0.072 |
F5+B6 recovered | Con A | 10 | 0.302 |
F5+B6 recovered | Con A | 20 | 0.329 |
F5+B6 recovered | MSP-119 | 10 | 0.338 |
F5+B6 recovered | MSP-119 | 20 | 0.445 |
Normal spleen corresponds to cells from naïve mice as negative control and cells stimulated with Con A are a positive control of proliferation/viability, detected in the MTS assay. A blank value of 0.147, obtained with normal spleen cells without stimulation with MSP-119 or ConA, was subtracted from each absorbance value. Each absorbance value represents the mean from three independent experiments.