Figure 2. ChIP display.

The image shows CRM2 elements labeled with P³³ on a polyacrylamide gel. The left panel shows results from chromatin immunoprecipitation with controls: pos, B73 nuclei used for the ChIP experiment; sup, supernatant that did not bind to CENH3 antibodies; C, CENH3-bound markers; neg, no antibody control (shows non-specific binding to the sepharose beads used for precipitation). The right panel shows an annotated comparison between sup and C lanes. The chromosomal locations of the bands precipitated are indicated. The dashes next to the S lane denote non-precipitated bands.