Table 2. List of primers and probes used in this study.
| Primers and probes | Sequence( 5′- 3′)d | Locatione | Length of the Amplicon(bp) | Comments |
| 3a-CFa | TGTAGCCGCTAAGCACTACCATCC | 15–38 | 275 | This pair of primers are used to clone sequence containing 3a sequence |
| 3a-CR | GGCAACAGCTCAACACCTTTGG | 268–289 | ||
| 3a-Fb | GGACGGCATCACGATTCTCT | 192–211 | 67 | This set of primers and probe are used in real-time PCR reaction of 3a. |
| 3a-R | CCTGAAAACTTGGCAGCAGTT | 238–258 | ||
| 3a-Tc | Fam-AAACGCCCTCGAATCGCTGGC-Tamra | 216–236 | ||
| caf1-CF | TTCCGTTATCGCCATTGCAT | 15–34 | 451 | This pair of primers are used to clone sequence containing caf1 sequence |
| caf1-CR | TGCAAGTTTACCGCCTTTGG | 446–465 | ||
| caf1-F | AGGTAAACGGTGAGAACCTTGTG | 365–387 | 78 | This set of primers and probe are used in real-time PCR reaction of caf1. |
| caf1-R | CAATTGAGCGAACAAAGAAATCC | 420–442 | ||
| caf1-T | Fam-ATGACGTCGTCTTGGCTACGGGCA-Tamra | 392–415 |
a
CF/CR:short for clone forward primer and clone reverse primer.
b
F/R:forward primer and reverse primer.
c
T:TaqMan probe.
d
FAM: 6-carboxy fluorescein; TAMRA: 6-carboxy-tetra-methylrhodamine.
e
Refererring to the positions in the corresponding sequences.