Table 5. C_t value for PCR reagent stored at 37°C for different days.

	Ct valuea													CVb
concentration	Day1	Day3	Day5	Day7	Day9	Day11	Day13	Day15	Day18	Day23	Day32	Day49	Day79
10	31.65	31.5	31.56	31.27	31.92	31.41	31.71	31.76	30.75	31.3	31.86	31.43	29.14	2.40
103	28.1	28.45	28.19	28.3	27.84	28.44	28.67	28.54	28.79	28.11	28.42	28.6	28.9	1.05
105	22.77	22.93	22.94	22.73	22.68	23.27	23.02	22.83	23.13	22.95	23.16	23.08	23.44	0.95
107	15.07	15.49	15.78	15.37	15.55	15.74	15.4	15.82	16.41	16.13	15.97	16.74	16.66	2.97

^aC_t: threshold cycle;values<35 are considered to be positive. Each C_t value is the mean result of the three values obtained each time point.

^bCV: coefficients of variations.