Skip to main content
. 2010 Mar 9;5(3):e9593. doi: 10.1371/journal.pone.0009593

Figure 2. Jak2 deficiency selectively attenuates the capacity of DCs to initiate innate immune response.

Figure 2

(A) Loss of Jak2 attenuates the capacity of DCs secretion of proinflammatory cytokines. Supernatants of BMDCs with or without LPS stimulation were harvested and subjected to ELISA analysis of cytokine secretion. BMDCs deficient for Jak2 showed significant reduced capacity to secrete TNFα and IL-12. (B) Jak2 failed to show perceptible impact on macrophages secretion of proinflammatory cytokines. PEM derived from Jak2−/− and control mice were cultured with or without LPS stimulation and supernatants were harvested for ELISA analysis as above, respectively. (C) Jak2−/− mice showed impaired early clearance of Listeria. Jak2−/− and control mice were i.v. injected with ∼1 LD50 Listeria Monocytogenes in PBS. Spleens and livers were harvested after 48 h infection. Serial 10-fold dilutions of organ homogenates were plated on TSB-agar and CFU were counted after 48 h incubation. Left panel: a representative results for cultures inoculated with homogenates; right panel: a bar graphic figure showing the average CFU/organ of 3 independent experiments. All data are present as mean ± SE. *, p<0.05; **, p<0.01; ***, p<0.001.