Skip to main content
. 2010 Mar 9;4(3):e625. doi: 10.1371/journal.pntd.0000625

Figure 9. Ecdysone-dependent transcriptional activation in B. malayi embryos.

Figure 9

(A) 20-E treatment causes a robust transcriptional activation with mediated by a consensus EcRE embedded in a B. malayi transcriptional reporter. Five copies of the EcRE used in the DNA binding assay (Fig. 4) were cloned in both orientations with respect to the direction of transcription in the BmRPS12-luciferase vector. Both reporters (EcRE and EcRE-rev) and the vector alone (dRPS12) were transformed into B. malayi embryos which were cultured in the presence of 20-E (+) or solvent alone (−) for 48 hrs and processed for luciferase assays. (B) 20-E causes a dose-response activation of the EcRE reporter. B. malayi embryos transformed with the BmRPS12- EcRE luciferase reporter were treated with the indicated concentrations of 20-OH ecdysone (in µM) or solvent alone (0). The luciferase activity obtained from solvent only treatment with the empty vector was set to 100%.