Table 2. Primers for Real-Time RT-PCR.
Primer | Accession No. | Orientation | Sequence (5′-3′) | Product Size (bp) |
GAPDH | NM002046 | Sense: | GGTGGTCTCCTCTGACTTCAACA | 127 |
Antisense: | GTTGCTGTAGCCAAATTCGTTGT | |||
HCV | NC004102 | Sense: | RAYCACTCCCCTGTGAGGAAC | 308 |
NC009823 | Antisense: | TGRTGCACGGTCTACGAGACCTC | ||
IFN-γ | NM000619 | Sense: | AGAAAAATAATGCAGAGCCAAATT | 275 |
Antisense: | TGACTCCTTTTTCGCTTCCCTGTT | |||
IL-12Rβ1 | NM153701 | Sense: | CCAGGAACCAGACAGAGAAG | 151 |
NM005535 | Antisense: | TCAGCACCAACCTGGTTATC | ||
IL-12Rβ2 | NM001559 | Sense: | ACATTCTTGGACATAGTGAGGCC | 80 |
Antisense: | GTACATCTGCTCACGGAAGCC | |||
Jak-2 | NM004972 | Sense: | TTTCGATGGATTTTGCCATT | 251 |
Antisense: | GCGAACAGTTTCCATCTGGT | |||
Tyk-2 | NM003331 | Sense: | CTTGACTGTGAACCGGGACT | 248 |
Antisense: | TGATGTGCTCGTGGTAGAGC | |||
STAT-1 | NM139266 | Sense: | GTGGAAAGACAGCCCTGCAT | 67 |
NM007315 | Antisense: | ACTGGACCCCTGTCTTCAAGAC | ||
STAT-3 | NM003150 | Sense: | GGCCCCTCGTCATCAAGA | 59 |
Antisense: | TTTGACCAGCAACCTGACTTTAGT | |||
STAT-4 | NM003151 | Sense: | CACCTGCCACATTGAGTCAACTA | 72 |
Antisense: | TAAGACCACGACCAACGTACGA | |||
STAT-5a, 5b | NM003152 | Sense: | AGATGCTGGCCGAGGTCAAC | 212 |
NM012448 | Antisense: | AGACTTGGCCTGCTGCTCAC | ||
SOCS-1 | NM003745 | Sense: | GACGCCTGCGGATTCTACTG | 138 |
Antisense: | GGCCATCTTCACGCTAAGGG | |||
SOCS-2 | NM003877 | Sense: | TGCAAGGATAAGCGGACAGG | 104 |
Antisense: | CAGAGATGCTGCAGAGATGG | |||
SOCS-3 | NM003955 | Sense: | TGCGCCTCAAGACCTTCAGC | 227 |
Antisense: | GATGCGCAGGTTCTTGGTCC | |||
PIAS-1 | NM016166 | Sense: | TCCCACCCAATCTTTGTGTG | 380 |
Antisense: | GCCGCATTTTACCAAGTGGA | |||
PIAS-3 | NM006099 | Sense: | TGCTGGCCGGAACAAGAGTG | 172 |
Antisense: | AGGGGGCAAAGAGAGAAGGG | |||
PIAS-x (a,b) | NM173206 | Sense: | TCTTCTGACGAAGAGGAAGACC | 275 |
NM004671 | Antisense: | TCAGAAGATGTTCCAAGCTTCA | ||
PIAS-y | NM015897 | Sense: | GAGAAGAAGCCCACCTGG A | 99 |
Antisense: | ACACTCGCTCAGGATCTTCG |