Figure 6. Visualizations into biochemical pathways.

Summary and visualizations into biochemical pathways: a) Effect of asphyxia on Krebs cycle intermediates; Fumarate, succinate and α-ketoglutarate are strongly increased after asphyxia, as well as lactate and alanine. These increases can be seen as the result of mitochondrial dysfunction and the subsequent disturbance of the Krebs cycle, since the Krebs cycle and mitochondrial function is coupled. b) Effect of different resuscitation protocols on these intermediates. Reoxygenation with 100% oxygen for either duration (g2/3) resulted in a significant delay in the decrease in fumarate, succinate and α-ketoglutarate compared to normoxic resuscitation (g1). The significantly lower levels of these Krebs cycle intermediates in the 21% FiO₂ resuscitation group indicates better mitochondrial recovery.