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. Author manuscript; available in PMC: 2011 Mar 26.
Published in final edited form as: J Mol Biol. 2010 Jan 25;397(2):575–586. doi: 10.1016/j.jmb.2010.01.043

Fig. 4.

Fig. 4

Single-step growth of KOS and vUL25-GFP. (a) The HSV-1 genome is represented at the top; UL and US refer to the long and short unique region sequences, respectively. The region of the HSV-1 genome that contains the UL25 gene is expanded below. The UL25-GFP fusion protein that is expressed from the vUL25-GFP virus is shown. (b) Replicate cultures of Vero cells were infected with KOS or vUL25-GFP at an MOI of 5. The cultures were harvested at the indicated times postinfection and freeze-thawed three times, and the yield of virus at each time point was determined by plaque titer determination on Vero cells.