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. Author manuscript; available in PMC: 2011 Apr 1.
Published in final edited form as: Virus Res. 2010 Jan 18;149(1):115–118. doi: 10.1016/j.virusres.2010.01.004

Fig. 1.

Fig. 1

(A) Entry of HSV-1 KOS UL45Δ into CHO-nectin-1 cells. HSV-1 KOS or KOS UL45Δ was added to CHO-nectin-1 cells at MOIs ranging from 0 to 2. Beta-galactosidase activity of cell lysates was quantified at 6 hr post-infection as an indication of viral entry. Entry of the two viruses was not statistically different (p = 0.40, Student’s t-test). (B) Effect of ammonium chloride on the cell entry of HSV-1 KOS UL45Δ. CHO-nectin-1 cells were treated with indicated concentration of ammonium chloride for 30 min. HSV-1 KOS or KOS UL45Δ (MOI of 1) was added to cells for 6 hr in the continued presence of agent. Entry was measured as the percent of beta-galactosidase activity of cell lysates relative to that obtained in the absence of lysosomotropic agent. Data are means of quadruplicate determinations with standard deviation. Entry was not statistically different in the presence of NH4Cl (p = 0.94, Student’s t-test).