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. Author manuscript; available in PMC: 2011 Apr 1.
Published in final edited form as: Mutat Res. 2010 Jan 8;686(1-2):1–8. doi: 10.1016/j.mrfmmm.2009.11.012

FIGURE 2. Parallel analysis of cultures of malignant cell lines using antibodies specific for transmembrane proteins or the FLAER reagent.

FIGURE 2

Samples were analyzed after expansion in vitro after sorting to eliminate preexisting mutants. Pseudo-density dot plots are shown for analyses using FITC-conjugated antibodies specific for transmembrane proteins (left panels) or the FLAER Alexa-488 reagent, which binds to the GPI structure directly (right panels). In all 3 examples, there are distinct populations of spontaneously arising cells that neither express GPI-linked proteins nor take up the FLAER reagent, but which do express transmembrane proteins. (A) A subclone of PR1-C (transformed lymphoma); (B) Jurkat, a T cell ALL cell line; (C) HBL2-A, derived from a Mantle Cell Lymphoma.