Table 1. Lymphoid neoplasms: determination of f and μ.
| Cell Type | Designation | * Starting # cells (× 10-6) | CE** | # days in culture¶ | # cell divisions, d¶¶ | # GPI(−) cells | Total # cells analyzed§ | Mutant frequency f (× 106)‡ | ‡‡ Mutation rate μ (× 107) |
|---|---|---|---|---|---|---|---|---|---|
| BLCL | Normal Donor | 1.0 | 20 | 6.42 | 13 | 1,191,119 | 11 | 17 | |
| Transformed Lymphoma | 0.57 | ||||||||
| PR1-C parental | .73 | 21 | 6.45 | 1760 | 1,052,108 | 1673 | 2593 | ||
| PR1-C-sub clone 1 | 2.08 | 21 | 5.44 | 2893 | 1,618,270 | 1787 | 3286 | ||
| PR1-C-sub clone 2 | 2.68 | 21 | 5.14 | 288 | 486,909 | 591 | 1151 | ||
| PR1-C-sub clone 3 | 2.49 | 21 | 5.82 | 1017 | 1,710,695 | 595 | 1022 | ||
| PR1-C-sub clone 4 | 2.06 | 21 | 5.27 | 2368 | 2,006,108 | 1180 | 2240 | ||
| Mantle Cell Lymphoma | 0.79 | ||||||||
| Experiment 1 | |||||||||
| HBL2- A | 1.4 | 21 | 6.23 | 1324 | 2,433,723 | 544 | 874 | ||
| Experiment 2 | |||||||||
| HBL2-A | 1.0 | 20 | 8.09 | 2236 | 2,274,028 | 983 | 1215 | ||
| Experiment 3 | |||||||||
| HBL2- A | 3.3 | 21 | 5.41 | 829 | 2,086,003 | 397 | 734 | ||
| HBL2- B | 3.8 | 26 | 6.17 | 952 | 2,913,268 | 326 | 529 | ||
| HBL2- C | 4.8 | 21 | 5.10 | 506 | 1,278,381 | 395 | 776 | ||
| T cell ALL | 0.93 | ||||||||
| Experiment 1 | |||||||||
| Jurkat Parental | 1.14 | 41 | 10.15 | 108 | 624,573 | 173 | 170 | ||
| Experiment 2 | |||||||||
| Jurkat Parental | 1.38 | 20 | 7.95 | 276 | 2,348,927 | 118 | 148 | ||
| Jurkat Clone 1 | 2.67 | 20 | 7.30 | 192 | 1,694,854 | 113 | 155 | ||
| Jurkat Clone 2 | 1.72 | 20 | 7.99 | 648 | 1,136,989 | 570 | 713 | ||
| Jurkat Clone 3 | 1.11 | 21 | 8.78 | 346 | 1,160,399 | 298 | 340 | ||
| Jurkat Clone 4 | 1.11 | 21 | 6.47 | 985 | 793,749 | 1240 | 1918 | ||
| Jurkat Clone 5 | 1.85 | 21 | 8.15 | 506 | 713,762 | 709 | 870 | ||
| Marginal Zone Lymphoma | SSK41 | 1.6 | 0.65 | 25 | 8.27 | 21 | 2,346,400 | 9 | 11 |
*
Number of GPI (+) cells collected by sorting, in millions
**
Cloning Efficiency in Bulk Culture
¶
Number of days in culture between sorting and analysis
¶¶
Number of cell divisions occurring in culture between sorting and analysis
§
Number of cells in the upper right quadrant plus number of cells in the lower right quadrant
‡
f = number of GPI (-) cells divided by total number of cells analyzed. Results expressed as mutants per 106 cells.
‡‡
Mutation rate calculated as μ= f divided by d. Results expressed as mutations per 107 cell divisions