Table 2. Plasma cell neoplasms: determination of f and μ.
| Cell line | Starting # cells (× 10-6)* | # days in culture¶ | CE** | # cell divisions, d¶¶ | # GPI(−) cells | Total # cells analyzed§ | Mutant frequency f (× 106)‡ | Mutation rate μ (× 107)‡‡ |
|---|---|---|---|---|---|---|---|---|
| KMS-11 | .38 | 22 | 0.56 | 6.74 | 5 | 1082484 | 4.62 | 6.9 |
| ARP-1 | 2.0 | 33 | 0.66 | 5.35 | 3 | 539077 | 5.57 | 10.4 |
| U266 | 6.3 | 33 | 0.69 | 5.00 | 62 | 1619500 | 38.3 | 76.6 |
| SKMM2 | 1.6 | 22 | 1.0 | 4.51 | 2 | 2027237 | 0.99 | 2.2 |
| NCI-H929 | 2.1 | 21 | 0.55 | 3.48 | 16 | 1235227 | 12.95 | 37.2 |
| ARH-77 | 7.3 | 19 | 0.82 | 3.21 | 82 | 1736463 | 47.2 | 147 |
| RMPI-8226 | 0.88 | 22 | 0.73 | 5.24 | 16 | 1487327 | 10.8 | 20.5 |
*
Number of GPI (+) cells collected by sorting, in millions
**
Cloning Efficiency in Bulk Culture
¶
Number of days in culture between sorting and analysis
¶¶
Number of cell divisions occurring in culture between sorting and analysis
§
Number of cells in the upper right quadrant plus number of cells in the lower right quadrant
‡
f = number of GPI (-) cells divided by total number of cells analyzed. Results expressed as mutants per 106 cells.
‡‡
Mutation rate calculated by the formula μ = f divided by d. Results expressed as mutations per 107 cell divisions