Fig. 6.

Swim path analysis of hidden platform acquisition trials. (A) Exposure to genistein during both gestation and lactation (G-G) significantly increased thigmotaxis on day 3 of training relative to controls. Asterisk indicates p < 0.05 by the Tukey post hoc test. Data are the mean ± SEM. (B) Swim path identification showed that rats exposed to genistein through both gestation and lactation (G-G) had their last day of relying on thigmotactic swimming significantly later than C-C rats. Asterisk indicates p = 0.007 (Mann-Whitney test). The bold horizontal line in each box indicates the median. Boxes extend to the 25^th and 75 percentiles. Whisker caps extend to the 10^th and 90^th percentiles. The data point is a subject that was below the 10th percentile. (C-D) Swim paths were identified and categorized for C-C (panel C) and G-G (panel D) rats as described in the Methods. The stacked bars in the graph show the percentage of rats that used thigmotaxis (black), non-spatial (white), or spatial (gray) strategies on the first trial of each training day. Note that on days 3 and 4 thigmotaxis is disappearing as a strategy to a greater extent in the C-C rats.