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. Author manuscript; available in PMC: 2011 Mar 13.
Published in final edited form as: Life Sci. 2010 Feb 8;86(11-12):448–454. doi: 10.1016/j.lfs.2010.01.017

Fig. 4.

Fig. 4

Removal of O2•− prevents [Zn2+]i elevation and reduces apoptotic cell death after UVB-irradiation. Panel A: NG-DCF fluorescence intensity was recorded from the cells that were treated with PEG-SOD from individual ROIs graphed as a function of time. The background lines represent control ROIs drawn in regions free of the cells. Panel B: The relative percentage of apoptotic cells with or without being pretreated with PEG-SOD after UVB-irradiation (50 mJ/cm2). The apoptotic cell death was analyzed at 24 h post-irradiation using the Annexin-V/PI double staining and flow cytometry method. The error bars represent the standard deviation of three independent experiments. * p < 0.05 vs. UVB alone. The lower panel shows the dot-plot graphs of flow cytometry.