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. Author manuscript; available in PMC: 2011 Mar 1.
Published in final edited form as: Int J Oral Maxillofac Surg. 2010 Jan 25;39(3):272–281. doi: 10.1016/j.ijom.2009.12.017

Table 1. Antibodies used in this study.

Antibody Antibody type Immunizing antigen Immunoreactivity in Western blotting Immunoreactivity in immunohistochemistry
Anti-DSP-2C12.3a Monoclonal Purified rat dentin DSP No Yes
Anti-DSPb Polyclonal Purified rat dentin DSP Yes Yes
Anti-DMP1-N-9B6.3c Monoclonal 37 kDa (N-terminal) Yes Very weak
Anti-DMP1-N-859d polyclonal Oligopeptide (residues 101-121) Yes Yes
Anti-DMP1-C-8G10.3e Monoclonal 57 kDa (C-terminal) No Yes
Anti-DMP1-C-857f Polyclonal Oligopeptide (residues 471-485) Yes Yes
Anti-BSP-10D9.2g Monoclonal Purified Rat bone BSP Yes Yes
Anti-OPNh Polyclonal Recombinant rat OPN Yes Yes
a

This monoclonal antibody1 was used to detect DSP by immunohistochemistry analysis.

b

This polyclonal antibody5 was used to detect DSP by Western immunoblotting.

c

This monoclonal antibody30 was used to detect the NH2-terminal fragment of DMP1 by Western immunoblotting analysis.

d

This polyclonal antibody21 was used to detect the NH2-terminal fragment of DMP1 by immunohistochemistry and immunofluorescence analysis.

e

This monoclonal antibody2 was used to detect the COOH-terminal fragment of DMP1 by immunohistochemistry and immunofluorescence analysis.

f

This polyclonal antibody15 was used to detect the COOH-terminal fragment of DMP1 by Western immunoblotting analysis.

g

This monoclonal antibody16 was used to detect BSP by immunohistochemistry analysis and Western immunoblotting.

h

This polyclonal antibody24 was used to detect OPN by immunohistochemistry analysis and Western immunoblotting.