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. Author manuscript; available in PMC: 2010 Oct 1.
Published in final edited form as: Dev Biol. 2010 Feb 1;340(1):67–74. doi: 10.1016/j.ydbio.2010.01.027

Fig. 3.

Fig. 3

Electrophoretic gel mobility shift assays on the mouse Pitx2 RAREs. (Lane 1) DR5a 31-mer probe incubated without nuclear extract added. (Lanes 2-3) DR5a and DR5a-mutant probes, respectively, incubated with E12.5 eye nuclear extract. (Lanes 4-6) DR5a probe supershifted by adding RAR antibodies along with the E12.5 eye nuclear extract. (Lanes 7-8) DR5b and DR5b-mutant probes, respectively, incubated with E12.5 eye nuclear extract.