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. Author manuscript; available in PMC: 2011 Jan 1.
Published in final edited form as: J Immunol. 2009 Nov 30;184(1):141–153. doi: 10.4049/jimmunol.0902880

Figure 7. Stability of glycolipid/CD1d complexes in vivo.

Figure 7

C57BL/6 mice were injected i.v. with 1 μg of the indicated glycolipids, and at the indicated times splenic CD11c+ DC were enriched by collagenase digestion and MACS bead isolation. The enriched DC or the DC-depleted cells were then cultured with the 1.2 Vα14i NKT hybridoma overnight, and IL-2 in the supernatant was assessed by ELISA. Results are plotted as the number of cells/well vs. IL-2 (Top panel: enriched DC. Bottom panel: DC-depleted cells). This experiment was performed twice with similar results.