Table 2.

Effects of glibenclamide and diazoxide on protein concentration, protein concentration/weight of slow-twitch SOL muscles and caspase-3 activity of control rats

	Control muscles	Contralateral control muscles after 72 h incubation with DMEM+	Muscles after 72 h incubation with DMEM+ and glibenclamide (10−6m)	Muscles after 72 h incubation with DMEM+, glibenclamide (10−6m) and diazoxide (10−4m)	Muscles after 72 h incubation with DMEM+ and diazoxide (10−4m)
Protein	2.11 ± 0.05	1.83 ± 0.06	1.64 ± 0.05*	1.75 ± 0.04	1.79 ± 0.03
concentration (μg μl−1)	(n= 7)	(n= 13)	(n= 7)	(n= 3)	(n= 3)
Protein concentration/	6.66 ± 0.03	5.56 ± 0.01	4.69 ± 0.01*	5.59 ± 0.01	5.69 ± 0.02
muscle weight (mg mg−1)	(n= 7)	(n= 13)	(n= 7)	(n= 3)	(n= 3)
Caspase-3 activity	0	1.33± 0.09	1.73 ± 0.09*	1.31 ± 0.08	1.32 ± 0.09
(10−6μmol pNA min−1 ml−1)	(n= 7)	(n= 13)	(n= 7)	(n= 3)	(n= 3)

Soleus (SOL) muscles from control rats were incubated for 72 h with the DMEM+, DMEM+ enriched with glibenclamide or diazoxide at 10⁻⁶m and 10⁻⁴m concentrations, respectively, under 5% CO₂ atmosphere at 37°C. Control muscles are samples that were rapidly processed after dissection, and therefore not exposed to DMEM+ or other DMEM+ drug-enriched solutions. n in parentheses indicates the number of SOL muscles sampled. Values are mean ± standard deviation.

^*Controls vs. all other groups (significantly different for P < 0.05 or less).