Table 1. Endogenous embryonic ecdysteroid composition: Percent of total Ecdysteroids (average of two replicates).
| Wild type
|
Homozygous shdz320
|
Heterozygous shdz320
|
|||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Age, h | VPP | 20E | E | 2dE | LPP | VPP | 20E | E | 2dE | LPP | VPP | 20E | E | 2dE | LPP |
| 0-2 | 0 | 0 | 3 | 97 | 0 | ||||||||||
| 2-4 | 0 | 0 | 20 | 80 | 0 | ||||||||||
| 4-6 | 0 | 0 | 50 | 50 | 0 | ||||||||||
| 6-8 | 5 | 10 | 72 | 10 | 3 | ||||||||||
| 10-12 | 85 | 5 | 0 | 0 | 10 | ||||||||||
| 12-14 | 87 | 3 | 0 | 0 | 10 | 4 | 0 | 45 | 50 | 1* | 85 | 8 | 0 | 0 | 7* |
Values were corrected for antibody cross-reactivity. In order of decreasing polarity: VPP, very polar products; 20E; E; 2dE; LPP, low-polarity products.
Ten to 14 h