Figure 2.

Ionizing radiation induces T cell specific differentiation in Ku80^−/− mice. Cells from different lymphoid organs from wild-type mice (Left), untreated Ku80^−/− mice (Center), and day 21 Ku80^−/− mice subjected to 50 cGy at birth (Right) were stained for the expression of various surface markers and analyzed by multiparameter flow cytometry. Thymocytes were analyzed for the expression of (A) CD4/CD8 and (B) T cell antigen receptor β(TCRβ) expression; splenocytes were stained for (C) CD4/CD8 and (E) CD43/B220; and bone marrow cells were analyzed for the expression of (D) IgM/B220. For data analysis, viable lymphoid cells were electronically gated according to forward and side scatter by using Cell-Quest software (Becton Dickinson). Data are plotted on a logarithmic scale except for B, which displays the cell counts on a linear scale.