Figure 3.

Representative western blot and RT-PCR analysis of biopsies from macaques transplanted with transduced hepatocytes. Western blot analysis using antibodies against EPO or tubulin, on protein extracts of (a) control nontransduced monkey liver and kidney. (b) Proteins were extracted from biopsies at 1 month before death (before GCV treatment) and (c) RNA was extracted from liver biopsies from different lobes before and at death (after GCV treatment). For RT analysis, the mTTR-cmEPO-TK vector plasmid was used as positive control. bp, base pair; EPO, erythropoietin; GCV, ganciclovir; TK, thymidine kinase.