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. 2009 Jul 14;17(10):1814–1821. doi: 10.1038/mt.2009.154

Figure 2.

Figure 2

Vesicular stomatitis virus (VSV) infection is limited to the olfactory bulbs following intranasal delivery. Mice received intranasal phosphate-buffered saline (PBS) or VSV-GFP in 10 µl PBS. Doses ranged from 1 × 106 to 1 × 109 plaque-forming units (pfu). Intact brains from mice euthanized 2, 5, 7, and 10 days postinfection were evaluated by fluorescence microscopy for evidence of GFP expression. Shown are representative images from mice treated with PBS (left panel) and 1 × 108 pfu VSV-GFP (right panel). (a) Olfactory nerves, 3 days postinfection. (b) Brains, 5 days postinfection. (c) Brains, 7 days postinfection. (d) Brains were harvested at 7, 14, and 28 days postinfection, fixed in formalin, embedded in paraffin, sectioned and stained with hematoxylin and eosin to look for signs of neurotoxicity. Representative sections from mice 28 days after receiving PBS (left panel) and 1 × 108 pfu VSV-GFP (right panel) are shown. GFP, green fluorescent protein.