Figure 3.

VSV-hDCT immunization elicits antigen-specific CD4⁺ and CD8⁺ T-cell responses. C57BL/6 mice (n = 5/treatment) were immunized with 1 × 10⁷ plaque-forming units (pfu) of VSV-hDCT. Negative and positive controls received PBS and 1 × 10⁸ pfu Ad-hDCT, respectively. Seven days later DCT-specific, blood-derived (a) CD4⁺ and (b) CD8⁺ T cells and (c) VSV-specific CD8⁺ T cells were quantified by flow cytometry after in vitro peptide restimulation and intracellular cytokine staining. Data shown are means plus standard error bars. Asterisks denote significant differences compared to PBS control (P ≤ 0.05, by one-way analysis of variance). Ad, adenovirus; hDCT, human dopachrome tautomerase; IFN-γ, interferon-γ; PBS, phosphate-buffered saline; VSV, vesicular stomatitis virus.