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. 2009 Jul 21;17(10):1804–1813. doi: 10.1038/mt.2009.165

Figure 7.

Figure 7

IMO does not alter T-cell memory populations and long-term response. (a) Memory T-cell analysis. Gating scheme was used in flow cytometry analysis. Cell numbers were characterized by FACS analysis of markers as described in the text. (b) Two monkeys per group were analyzed 4 months after the last Ad6 injection. The bars indicate CD95− (naive); CD95+, CD28−, and CD45RA− (effector memory, EM); CD95+, CD28−, and CD45RA+ (terminally differentiated effector memory, TDEM); CD95+, CD28+, and CD45RA− (central memory 1, CM1); CD95+, CD28+, and CD45RA+ (central memory 2, CM2). Asterisks indicate time points where differences are statistically significant (P < 0.05; Student's t-test). (c) A single DNA-EP boost 4 months after the second Ad6 injection restores the T-cell response. Groups of four monkeys were treated with vaccine alone (Vax) or combined with IMO (Vax + 2.5 mg IMO) were treated by DNA-EP at the indicated time point. Open circles indicate individual animals, whereas filled circles are the group averages. The total reactivity for human telomerase reverse transcriptase peptide pools was calculated as before. Ad6, adenovirus serotype 6; CM, central memory; DNA-EP, DNA electroporation; IMO, immunomodulatory oligonucleotide; PBMC, peripheral blood mononuclear cell; SFC, spot-forming cell; Vax, vaccination.