Skip to main content
. 2009 Aug 11;17(10):1712–1723. doi: 10.1038/mt.2009.176

Figure 6.

Figure 6

The e-shRNA and lhRNA transcripts do not induce the interferon response. Total RNA was purified from HEK 293T cells transfected with (a) e-shRNAs and (b) lhRNAs at an efficacious dose. The IFN-β, OAS, RIG-I, ISG56, STAT-1, and MxA expression levels were determined by RT-PCR. β-actin mRNA expression serves as an internal control. We included poly I:C transfected cells as positive control. As negative controls, cells were Mock transfected or with the pSUPER vector (ctr). We also performed a control PCR on RNA extracts derived from poly I:C transfected cells that were not subjected to reverse transcription. Either the 200 or 400 bp lane of the SmartLadder (M) (Eurogentec) is shown as size reference. HEK, human embryonic kidney; IFN-β, interferon-β; lhRNA, long hairpin RNA; MxA, myxovirus resistant gene A; OAS, 2′-5′ oligoadenylate synthase; RIG-I, retinoic acid-inducible gene-I; shRNA, short hairpin RNA; STAT-1, signal transduction and activator of transcription-1.