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. 2009 Aug 11;17(10):1712–1723. doi: 10.1038/mt.2009.176

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Copyright 2009, The American Society of Gene & Cell Therapy

This work is licensed under the Creative Commons Attribution-NonCommercial-No Derivative Works 3.0 License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/

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Suppression of HIV-1 replication in e3-66 expressing T cells. SupT1 cells were transduced with the lentiviral vector encoding e3-66 shNef, shPol, or shR/T. The empty JS1 vector was used as a negative control. (a) Transduced GFP-positive cells were selected by sorting and infected with low (left panel) and high (right panel) virus input. Virus replication was monitored for 49 days by measuring CA-p24 in the culture supernatant. (b) Clonal sequencing reveals the genetic alterations in the three target regions in different viral clones. GFP, green fluorescent protein; HIV, human immunodeficiency virus.