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. 2009 Jul 14;17(11):1967–1978. doi: 10.1038/mt.2009.151

Figure 2.

Figure 2

Characterization of transduced hBMSCs in vitro. (a) Influence of lentiviral transduction on hBMSCs. BrdU incorporation assay was used to assess the proliferation of both transduced and nontransduced cells by immunocytochemistry (top panel) and FACS analysis (bottom panel). The numbers indicate the percentage of BrdU+ cells. Original magnification: ×40. (b). Analysis of the differentiation potential of transduced hMSCs by RT-PCR. GAPDH was used as a house-keeping gene. (c) von Kossa staining and Oil Red O staining of nontransduced and transduced cells after 4-week induction of osteogenic differentiation or adipogenic differentiation. ALP, alkaline phosphatase; BrdU, 5′-bromo-2-deoxyuridine; BSP, bone sialoprotein; Col1A1, collagen 1A1; FACS, fluorescence-activated cell-sorting; GADPH, glyceraldehyde-3-phosphate dehydrogenase; GFP, green fluorescent protein; hBMSCs, human bone marrow stromal cells; OI, osteogenic induction.